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Characterization of a new class of thermophilic pullulanases from Bacillus acidopullulyticus.

作者信息

Schülein M, Højer-Pedersen B

出版信息

Ann N Y Acad Sci. 1984;434:271-4. doi: 10.1111/j.1749-6632.1984.tb29842.x.

DOI:10.1111/j.1749-6632.1984.tb29842.x
PMID:6596899
Abstract
摘要

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Improving the thermostability and catalytic efficiency of Bacillus deramificans pullulanase by site-directed mutagenesis.通过定点突变提高解淀粉芽孢杆菌普鲁兰酶的热稳定性和催化效率。
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Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli.
在嗜热古菌沃氏嗜热栖热菌（Pyrococcus woesei）的基因在大肠杆菌中克隆和表达后，对其热稳定支链淀粉酶进行分离和特性鉴定。
Appl Environ Microbiol. 1995 Feb;61(2):567-75. doi: 10.1128/aem.61.2.567-575.1995.
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Characterization of alpha-amylase and pullulanase activities of Clostridium thermohydrosulfuricum. Evidence for a novel thermostable amylase.嗜热栖热硫化叶菌α-淀粉酶和支链淀粉酶活性的表征。一种新型耐热淀粉酶的证据。
Biochem J. 1987 Aug 15;246(1):193-7. doi: 10.1042/bj2460193.
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Cloning of the debranching-enzyme gene from Thermoanaerobium brockii into Escherichia coli and Bacillus subtilis.将嗜热栖热放线菌的脱支酶基因克隆到大肠杆菌和枯草芽孢杆菌中。
J Bacteriol. 1987 Sep;169(9):4302-7. doi: 10.1128/jb.169.9.4302-4307.1987.
6
A hyperthermostable pullulanase produced by an extreme thermophile, Bacillus flavocaldarius KP 1228, and evidence for the proline theory of increasing protein thermostability.嗜热栖热菌Bacillus flavocaldarius KP 1228产生的一种超嗜热支链淀粉酶以及蛋白质热稳定性增加的脯氨酸理论的证据。
Appl Microbiol Biotechnol. 1991 Mar;34(6):707-14. doi: 10.1007/BF00169338.