Moe D, Salling E, Kirkeby S
Scand J Dent Res. 1984 Dec;92(6):503-7. doi: 10.1111/j.1600-0722.1984.tb01289.x.
Two multi-step extraction procedures were used to isolate matrix proteins from fetal bovine enamel. Using SDS-PAGE and gel isoelectric focusing it was concluded that extraction procedures influence the number of protein bands in the gel, their pI, apparent molecular weight and staining intensity.
采用两种多步提取程序从胎牛牙釉质中分离基质蛋白。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和凝胶等电聚焦分析得出,提取程序会影响凝胶中蛋白条带的数量、其等电点、表观分子量和染色强度。