Denny P C, Denny P A
Arch Oral Biol. 1984;29(12):1033-40. doi: 10.1016/0003-9969(84)90151-1.
The validity of a radioimmunoassay (RIA) for the predominant sialomucin from female mouse submandibular glands was confirmed by immunological and non-immunological approaches. The maximum sensitivity was in the low nanogramme range. The RIA did not cross-react with parotid, sublingual, trachea, stomach or colon tissues, but showed incomplete cross-reactivity with duodenal tissue. An analysis of protein, sialic acid and mucin contents of submandibular glands obtained during diurnal samplings, indicated that mean sialic acid and mucin concentrations, relative to protein in sample groups, decreased overnight approx. 30 per cent and showed a net increase during the day. Sialic acid per gland showed a 3-fold variation among individuals in the population; mucin showed greater than 20-fold variation. An analysis of variation indicated that influence by time of day was the greatest factor affecting sialic-acid concentrations and was statistically significant but, variation in mucin concentration was not. Variation between individuals accounted for more than 90 per cent of the total population variation in mucin concentrations and in sialic acid to mucin ratios. Regression analyses of the sialic acid to mucin ratios at different times of day suggested that the rates of replenishment of sialic acid and mucin may be co-ordinated.
通过免疫学和非免疫学方法证实了一种用于检测雌性小鼠下颌下腺中主要涎粘蛋白的放射免疫测定法（RIA）的有效性。最大灵敏度在低纳克范围内。该放射免疫测定法与腮腺、舌下腺、气管、胃或结肠组织无交叉反应，但与十二指肠组织存在不完全交叉反应。对昼夜采样期间获得的下颌下腺的蛋白质、唾液酸和粘蛋白含量进行分析，结果表明，相对于样本组中的蛋白质，唾液酸和粘蛋白的平均浓度在夜间约降低30%，并在白天呈现净增加。每个腺体的唾液酸在群体中的个体间存在3倍的差异；粘蛋白的差异大于20倍。变异分析表明，一天中的时间影响是影响唾液酸浓度的最大因素，且具有统计学意义，但粘蛋白浓度的变异则不然。个体间变异占粘蛋白浓度以及唾液酸与粘蛋白比率的总群体变异的90%以上。对一天中不同时间的唾液酸与粘蛋白比率进行回归分析表明，唾液酸和粘蛋白的补充速率可能是协调的。
