Diurnal variation of sialomucin concentration in female mouse submandibular glands measured by radioimmunoassay.

The validity of a radioimmunoassay (RIA) for the predominant sialomucin from female mouse submandibular glands was confirmed by immunological and non-immunological approaches. The maximum sensitivity was in the low nanogramme range. The RIA did not cross-react with parotid, sublingual, trachea, stomach or colon tissues, but showed incomplete cross-reactivity with duodenal tissue. An analysis of protein, sialic acid and mucin contents of submandibular glands obtained during diurnal samplings, indicated that mean sialic acid and mucin concentrations, relative to protein in sample groups, decreased overnight approx. 30 per cent and showed a net increase during the day. Sialic acid per gland showed a 3-fold variation among individuals in the population; mucin showed greater than 20-fold variation. An analysis of variation indicated that influence by time of day was the greatest factor affecting sialic-acid concentrations and was statistically significant but, variation in mucin concentration was not. Variation between individuals accounted for more than 90 per cent of the total population variation in mucin concentrations and in sialic acid to mucin ratios. Regression analyses of the sialic acid to mucin ratios at different times of day suggested that the rates of replenishment of sialic acid and mucin may be co-ordinated.