Howerton T C, Collins A C
Alcohol. 1984 Nov-Dec;1(6):471-7. doi: 10.1016/0741-8329(84)90024-7.
The effects of ethanol on K+-stimulated and spontaneous release of gamma-aminobutyric acid (GABA) were studied in cortical and cerebellar brain slices obtained from long-sleep (LS) and short-sleep (SS) mice. A superfusion technique was used. Tissue slices were perfused with ethanol (0-515 mM) for 20 min. Ethanol inhibited K+-stimulated 3H-GABA release at lower concentrations in LS cortical slices than in SS slices. Little or no inhibition of K+-stimulated 3H-GABA release was seen in both LS and SS cerebellar slices. The spontaneous release of 3H-GABA was inhibited to equal degrees in LS and SS cerebellar slices but was unaffected in cortical slices, at the concentrations used. The possibility that ethanol inhibits 3H-GABA release by stimulating prostaglandin production was partially tested by assessing the effects of prostaglandin F2 alpha (PGF2 alpha) on 3H-GABA release in cortical and cerebellar slices. No effect of PGF2 alpha on 3H-GABA release was seen in either brain region. These results support the notion that ethanol may elicit some of its actions by inhibiting neurotransmitter release. This effect appears to be influenced by genotype and brain region.
研究了乙醇对从长睡眠（LS）和短睡眠（SS）小鼠获取的大脑皮层和小脑脑片钾离子刺激型及自发性γ-氨基丁酸（GABA）释放的影响。采用了灌流技术。用乙醇（0 - 515 mM）对组织切片灌流20分钟。在较低浓度下，乙醇抑制LS大脑皮层切片中钾离子刺激型3H - GABA的释放比抑制SS切片中的更明显。在LS和SS小脑脑片中，几乎未观察到乙醇对钾离子刺激型3H - GABA释放的抑制作用。在所使用的浓度下，LS和SS小脑脑片中3H - GABA的自发性释放受到同等程度的抑制，但在大脑皮层切片中未受影响。通过评估前列腺素F2α（PGF2α）对大脑皮层和小脑脑片中3H - GABA释放的影响，部分测试了乙醇通过刺激前列腺素生成来抑制3H - GABA释放的可能性。在任一脑区均未观察到PGF2α对3H - GABA释放的影响。这些结果支持乙醇可能通过抑制神经递质释放来引发其某些作用的观点。这种作用似乎受基因型和脑区的影响。
