Taetle R, Caviles A, Koziol J
Cancer Res. 1983 May;43(5):2350-7.
The response of human myeloid leukemia cells to various sources of colony-stimulating activity (CSA) and media conditioned by phytohemagglutinin-stimulated mononuclear cells (PHA-LCM) was investigated in liquid and colony culture. PHA-LCM, placenta-conditioned medium, GCT cell line-conditioned medium, leukocyte-conditioned medium, and partially purified CSA for human and murine cells were tested for ability to support growth of granulocyte-macrophage colonies from adherent cell-depleted human bone marrow. This activity was correlated with ability to support leukemia colony growth in methylcellulose, and [3H]thymidine incorporation in liquid culture by normal bone marrow cells, leukemia cells, and the KG-1 myeloid leukemia cell line. For normal cells, growth and liquid culture responses were highly correlated for various sources of CSA (r = 0.92), and addition of data using PHA-LCM changed results only slightly (r = 0.89). [3H]thymidine incorporation by leukemia cells from patients without a prior history of a myeloproliferative disorder was also highly correlated with normal CSA (r = 0.97) for sources other than PHA-LCM. Responses of leukemia blasts and KG-1 cells in liquid culture to PHA-LCM appeared in excess of its CSA for normal cells. Colony growth by leukemia cells was not clearly correlated with either liquid culture activity for leukemia cells or CSA for normal cells. PHA-LCM was also not statistically superior to placenta-conditioned medium as stimulus for leukemia colony growth, but was superior to placenta-conditioned medium for some patients. Differentiation in culture did not appear to depend on CSA source. We conclude that normal myeloid cells respond to CSA in a highly correlated fashion in both colony and liquid cultures. The majority of myeloid leukemia cells respond to either PHA-LCM or CSA, but the ability of PHA-LCM to support leukemia cell growth is greater than its CSA content. The possibility exists that overlapping populations responsive to CSA and to PHA-LCM are present simultaneously in patients with myeloid leukemia.
在液体培养和集落培养中，研究了人髓系白血病细胞对各种集落刺激活性（CSA）来源以及由植物血凝素刺激的单核细胞（PHA-LCM）条件培养基的反应。测试了PHA-LCM、胎盘条件培养基、GCT细胞系条件培养基、白细胞条件培养基以及人源和鼠源细胞的部分纯化CSA，以评估其支持去除贴壁细胞的人骨髓中粒细胞-巨噬细胞集落生长的能力。这种活性与支持甲基纤维素中白血病集落生长的能力以及正常骨髓细胞、白血病细胞和KG-1髓系白血病细胞系在液体培养中[3H]胸苷掺入相关。对于正常细胞，各种CSA来源的生长和液体培养反应高度相关（r = 0.92），使用PHA-LCM添加数据后结果仅略有变化（r = 0.89）。对于除PHA-LCM以外的其他来源，无骨髓增殖性疾病既往史患者的白血病细胞[3H]胸苷掺入也与正常CSA高度相关（r = 0.97）。液体培养中白血病原始细胞和KG-1细胞对PHA-LCM的反应似乎超过了其对正常细胞的CSA。白血病细胞的集落生长与白血病细胞的液体培养活性或正常细胞的CSA均无明显相关性。PHA-LCM作为白血病集落生长的刺激物在统计学上也并不优于胎盘条件培养基，但对某些患者优于胎盘条件培养基。培养中的分化似乎不依赖于CSA来源。我们得出结论，正常髓系细胞在集落培养和液体培养中对CSA的反应高度相关。大多数髓系白血病细胞对PHA-LCM或CSA有反应，但PHA-LCM支持白血病细胞生长的能力大于其CSA含量。髓系白血病患者中可能同时存在对CSA和PHA-LCM有反应的重叠群体。
