Bertschmann M, Lüscher E F
Cell Immunol. 1983 May;78(1):13-22. doi: 10.1016/0008-8749(83)90255-1.
Cells from the draining lymph nodes of DBA/2 mice bearing syngeneic intradermal P-815 tumors represent an excellent responding cell population for secondary stimulation in vitro, despite the virtual absence of response by spleen cells of the same animals. Cytotoxicity is a result of stimulation with intact mitomycin-treated tumor cells. Isolated tumor cell membranes, in the form of small vesicles, stimulated cytotoxicity to a very limited extent and inhibited the development of cytotoxic T lymphocytes over a wide range of concentrations. Membrane proteins solubilized with deoxycholate and papain had only a suppressive effect. Soluble proteins exerted their effect during the induction of cytotoxic T lymphocytes and were ineffective when present during the effector phase of cytotoxic T lymphocytes. The suppressive capacity was shown to reside in a cell population which was sensitive to treatment with monoclonal Lyt-2.1 antibody and complement but not with Lyt-1.1 antibody. This phenotype was compatible with a specific rather than a promiscuous type of suppressor effector cell.
携带同基因皮内P - 815肿瘤的DBA/2小鼠引流淋巴结中的细胞，是体外二次刺激的优良反应细胞群体，尽管相同动物的脾细胞几乎没有反应。细胞毒性是完整的丝裂霉素处理的肿瘤细胞刺激的结果。以小泡形式存在的分离肿瘤细胞膜在非常有限的程度上刺激细胞毒性，并在很宽的浓度范围内抑制细胞毒性T淋巴细胞的发育。用脱氧胆酸盐和木瓜蛋白酶溶解的膜蛋白只有抑制作用。可溶性蛋白在细胞毒性T淋巴细胞诱导过程中发挥作用，而在细胞毒性T淋巴细胞效应期存在时则无效。抑制能力显示存在于对单克隆Lyt - 2.1抗体和补体治疗敏感但对Lyt - 1.1抗体不敏感的细胞群体中。这种表型与特异性而非混杂型抑制效应细胞相符。
