人脑脊液中免疫反应性促肾上腺皮质激素释放因子的存在

Presence of immunoreactive corticotropin-releasing factor in human cerebrospinal fluid.

作者信息

Suda T, Tozawa F, Mouri T, Demura H, Shizume K

出版信息

J Clin Endocrinol Metab. 1983 Jul;57(1):225-6. doi: 10.1210/jcem-57-1-225.

Abstract

Immunoreactive corticotropin-releasing factor (I-CRF) was measured by radioimmunoassay and immunoaffinity chromatography in human hypothalamus and cerebrospinal fluid (CSF). Dilution curves of I-CRF in the hypothalamus and CSF were parallel to that of synthetic ovine CRF standard. I-CRF content in the hypothalamus was 643 and 281 fmol eq, respectively. I-CRF concentration in CSF was 7.4 +/- 1.1 fmol eq/ml. Sephadex G-75 column chromatography showed the main peak eluted at the position of synthetic CRF.

摘要

采用放射免疫分析法和免疫亲和色谱法测定人下丘脑和脑脊液（CSF）中的免疫反应性促肾上腺皮质激素释放因子（I-CRF）。下丘脑和脑脊液中I-CRF的稀释曲线与合成羊CRF标准品的稀释曲线平行。下丘脑I-CRF含量分别为643和281 fmol当量。脑脊液中I-CRF浓度为7.4±1.1 fmol当量/毫升。葡聚糖凝胶G-75柱色谱显示主峰在合成CRF的位置洗脱。