Smith T, Chisholm G D, Habib F K
J Steroid Biochem. 1983 May;18(5):531-4. doi: 10.1016/0022-4731(83)90127-9.
The effect of sodium molybdate and the stability of [3H]-R1881 on the reproducibility of androgen receptor assays in the human prostate gland have been investigated. The drying down of radio-labelled methyltrienolone, prior to incubation with cytosolic and nuclear extracts, resulted in a marked disintegration of the compound and a loss of specific binding of up to 40%. By contrast, the inclusion of sodium molybdate in the homogenisation buffer significantly increased the specific nuclear and cytosolic binding. In the cytosol, this increase was directly proportional to the amount of molybdate added up to 50 mM whereas in the nucleus binding was maximal at a molybdate concentration of 10 mM and declined at higher concentrations.
研究了钼酸钠的作用以及[3H]-R1881的稳定性对人前列腺雄激素受体检测重现性的影响。在与胞质和核提取物孵育之前，放射性标记的甲基三烯醇酮干燥后，该化合物出现明显分解，特异性结合损失高达40%。相比之下，在匀浆缓冲液中加入钼酸钠可显著增加核和胞质的特异性结合。在胞质中，这种增加与添加的钼酸盐量直接成正比，最高可达50 mM，而在细胞核中，钼酸盐浓度为10 mM时结合量最大，浓度更高时则下降。
