Baird A, Kan K W, Solomon S
J Steroid Biochem. 1983 May;18(5):581-4. doi: 10.1016/0022-4731(83)90134-6.
The possibility that the formation of androstenedione by fetal calf adrenal cells in culture is linked to their decreased ability to form cortisol and corticosterone was investigated. Fetal calf adrenal cells metabolise radioactive adrostenedione to two major products which coelute on thin layer chromatography with 11 beta-hydroxyandrostenedione and 11 beta-hydroxytestosterone. When the cells are incubated with 11-deoxycortisol or 11-deoxycorticosterone in the presence of androstenedione there is a dose dependant inhibition of cortisol and corticosterone formation. Further studies with progesterone showed an accumulation of 11-deoxycortisol and 11-deoxycorticosterone in cells incubated simultaneously with androstenedione. The results demonstrate that exogenous androstenedione can have dramatic effects on steroidogenesis in the fetal calf adrenal and suggest that the accumulation of androstenedione in the medium of cultured andrenocortical cells is responsible, at least in part, for the decreased formation of cortisol and corticosterone.
研究了培养的胎牛肾上腺细胞中雄烯二酮的形成与其生成皮质醇和皮质酮能力下降之间的关联。胎牛肾上腺细胞将放射性雄烯二酮代谢为两种主要产物，这两种产物在薄层色谱上与11β-羟基雄烯二酮和11β-羟基睾酮共洗脱。当细胞在雄烯二酮存在的情况下与11-脱氧皮质醇或11-脱氧皮质酮一起孵育时，皮质醇和皮质酮的形成受到剂量依赖性抑制。对孕酮的进一步研究表明，在与雄烯二酮同时孵育的细胞中，11-脱氧皮质醇和11-脱氧皮质酮会积累。结果表明，外源性雄烯二酮可对胎牛肾上腺的类固醇生成产生显著影响，并表明培养的肾上腺皮质细胞培养基中雄烯二酮的积累至少部分导致了皮质醇和皮质酮形成的减少。
