体液因子在粒细胞生成中的作用：集落促进因子（CPF）及其在长期骨髓培养中的靶细胞“前CFU-c”
Role of humoral factors in granulopoiesis: colony promoting factor (CPF) and its target 'pre-CFU-c' in long-term bone marrow culture.
作者信息
Izumi H, Tsurusawa M, Miyanomae T, Kumagai K, Mori K J
出版信息
Leuk Res. 1983;7(2):155-65. doi: 10.1016/0145-2126(83)90006-1.
Supernatant of long-term bone marrow cultures contains colony promoting factor (CPF). The factor has no colony stimulating activity itself but augments granulocyte-macrophage colony formation in semi-solid culture of bone marrow cells in the presence of colony stimulating factor (CSF). CPF was specifically absorbed by bone marrow cells but not by thymocytes. Incubation of bone marrow cells with CPF alone resulted in the increase of the number of granulocyte-macrophage progenitor cells (CFUc). CPF-containing supernatant did not stimulate the proliferation of pluripotent stem cells (CFUs) but inhibited CFUs proliferation. These results suggest that CPF is a separate factor from CSF or from CFUs regulators, and that CSF-non-responsive cells (pre-CFUc) in the bone marrow mature into CSF-responsive CFUc by the stimulus of CPF.
长期骨髓培养物的上清液含有集落促进因子（CPF）。该因子本身没有集落刺激活性，但在集落刺激因子（CSF）存在的情况下，可增强骨髓细胞半固体培养中的粒细胞-巨噬细胞集落形成。CPF被骨髓细胞特异性吸收，但不被胸腺细胞吸收。单独用CPF孵育骨髓细胞导致粒细胞-巨噬细胞祖细胞（CFUc）数量增加。含CPF的上清液不刺激多能干细胞（CFUs）的增殖，但抑制CFUs的增殖。这些结果表明，CPF是一种与CSF或CFUs调节因子不同的因子，并且骨髓中对CSF无反应的细胞（前CFUc）通过CPF的刺激成熟为对CSF有反应的CFUc。
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