Zagury D, Bernard J, Morgan D A, Fouchard M, Feldman M
Int J Cancer. 1983 Jun 15;31(6):705-10. doi: 10.1002/ijc.2910310605.
Human normal T cells were selected for in vitro cloning according to the expression of T4, T8 or T10 antigens on individual cells. Clones were produced from each of these cells irrespective of the antigenic phenotype of the parental cell. The cloned progeny manifested, in many cases, shifts in antigen expression. Thus, T4+T8- cells gave clones expressing predominantly T4-T8+ and vice versa. The clonal expression of T4 and T8 seemed to be mutually exclusive. Antigenic shifts were recorded also in clones derived from T4-T8-T10- cells, resulting in T10+ clones which were also either T4+ or T8+ and from T4+T8-T10+ cloned cells yielding clones of either T4+ or T8+ cells. Testing functional properties we found that NK activity was mediated not only by T10+ cells but also, in some cases, by T4+ and T8+ cells. Moreover, TCGF production, which may reflect helper activity, was mediated not only by T4+ cells. Only the cytotoxic (CTL) activity seems to be confined to the T8 phenotype. Thus, it appears that T antigens, which seemed to be molecular markers of differentiation, are not markers for terminal differentiation and do not always reflect defined functional properties. These conclusions are drawn from cloning of normal T cells which manifest properties different from those of T-cell lines or T hybridomas.
根据单个细胞上T4、T8或T10抗原的表达情况，选择人正常T细胞进行体外克隆。从这些细胞中的每一个产生克隆，而不考虑亲代细胞的抗原表型。在许多情况下，克隆后代表现出抗原表达的变化。因此，T4+T8-细胞产生的克隆主要表达T4-T8+，反之亦然。T4和T8的克隆表达似乎相互排斥。在源自T4-T8-T10-细胞的克隆中也记录到抗原变化，产生T10+克隆，这些克隆也是T4+或T8+，并且从T4+T8-T10+克隆细胞产生T4+或T8+细胞的克隆。测试功能特性时，我们发现NK活性不仅由T10+细胞介导，在某些情况下还由T4+和T8+细胞介导。此外，可能反映辅助活性的TCGF产生不仅由T4+细胞介导。只有细胞毒性（CTL）活性似乎局限于T8表型。因此，似乎T抗原，其似乎是分化的分子标记，不是终末分化的标记，并且并不总是反映明确的功能特性。这些结论来自正常T细胞的克隆，其表现出与T细胞系或T杂交瘤不同的特性。
