Luger T A, Charon J A, Colot M, Micksche M, Oppenheim J J
J Immunol. 1983 Aug;131(2):816-20.
Human epidermal cell thymocyte-activating factor (ETAF) derived from either normal epidermal cells or a squamous cell carcinoma cell line has recently been shown to be a low m.w. protein that is indistinguishable from human macrophage-derived interleukin 1 (IL 1). As with human IL 1, human ETAF elutes from Sephadex S-200 gel in two major peaks at m.w. 70,000 to 40,000 and m.w. 25,000 to 12,000. Rechromatography of the higher m.w. fraction of ETAF yielded some of the lower m.w. activity, and chromatofocusing of high m.w. ETAF yielded the same three isoelectric points as the lower m.w. ETAF. Partially purified human ETAF as well as IL 1 were chemotactic for polymorphonuclear and mononuclear leukocytes. In addition, exposure of PMN to ETAF stimulated increased metabolic activity as demonstrated by greater reduction of intracellular nitroblue tetrazolium. Therefore, this study lends further support for an important role of ETAF in the pathogenesis of inflammatory skin diseases.
最近研究表明，源自正常表皮细胞或鳞状细胞癌细胞系的人表皮细胞胸腺细胞激活因子（ETAF）是一种低分子量蛋白质，与人巨噬细胞衍生的白细胞介素1（IL-1）无法区分。与人IL-1一样，人ETAF从Sephadex S-200凝胶中洗脱时出现两个主要峰，分子量分别在70,000至40,000以及25,000至12,000之间。对ETAF较高分子量部分进行再色谱分析可得到一些较低分子量的活性成分，对高分子量ETAF进行色谱聚焦可得到与低分子量ETAF相同的三个等电点。部分纯化的人ETAF以及IL-1对多形核白细胞和单核白细胞具有趋化作用。此外，将多形核白细胞暴露于ETAF可刺激其代谢活性增强，这可通过细胞内硝基蓝四氮唑的还原程度更高得到证明。因此，本研究进一步支持了ETAF在炎症性皮肤病发病机制中起重要作用的观点。
