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Clonal analysis of cytotoxic T cell response against human melanoma.针对人类黑色素瘤的细胞毒性T细胞反应的克隆分析。
J Exp Med. 1983 Jul 1;158(1):240-5. doi: 10.1084/jem.158.1.240.
2
Clonal analysis of cytotoxic and regulatory T cell responses against human melanoma.针对人类黑色素瘤的细胞毒性和调节性T细胞反应的克隆分析。
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Activation of autoreactive cytolytic T lymphocyte clone against human melanoma by anti-T3 monoclonal antibody and autologous accessory cells.抗T3单克隆抗体和自体辅助细胞激活针对人黑色素瘤的自身反应性溶细胞性T淋巴细胞克隆
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Cloned human cytotoxic T lymphocyte (CTL) lines reactive with autologous melanoma cells. I. In vitro generation, isolation, and analysis to phenotype and specificity.与自体黑色素瘤细胞反应的克隆化人细胞毒性T淋巴细胞（CTL）系。I. 体外生成、分离及表型和特异性分析。
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Selective expansion of a specific anti-tumor CD8+ cytotoxic T lymphocyte clone in the bulk culture of tumor-infiltrating lymphocytes from a melanoma patient: cytotoxic activity and T cell receptor gene rearrangements.在一名黑色素瘤患者肿瘤浸润淋巴细胞的大量培养物中特定抗肿瘤CD8 + 细胞毒性T淋巴细胞克隆的选择性扩增：细胞毒性活性和T细胞受体基因重排
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Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.针对自体人黑色素瘤的细胞溶解性T细胞克隆：特异性研究及通过免疫选择对三种抗原的定义
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Tumor-specific T-cell clones recognize different protein determinants of autologous human malignant melanoma cells.肿瘤特异性T细胞克隆识别自体人类恶性黑色素瘤细胞的不同蛋白质决定簇。
Int J Cancer. 1990 May 15;45(5):834-41. doi: 10.1002/ijc.2910450508.
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Human T cell clones exerting multiple cytolytic activities show heterogeneity in susceptibility to inhibition by monoclonal antibodies.表现出多种细胞溶解活性的人T细胞克隆在对单克隆抗体抑制的敏感性方面存在异质性。
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An efficient strategy to induce and maintain in vitro human T cells specific for autologous non-small cell lung carcinoma.一种高效的策略，用于诱导和维持体外针对自体非小细胞肺癌的人 T 细胞特异性。
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A peptide recognized by human cytolytic T lymphocytes on HLA-A2 melanomas is encoded by an intron sequence of the N-acetylglucosaminyltransferase V gene.一种在HLA - A2黑色素瘤上被人细胞溶解性T淋巴细胞识别的肽由N - 乙酰葡糖胺基转移酶V基因的一个内含子序列编码。
J Exp Med. 1996 Mar 1;183(3):1173-83. doi: 10.1084/jem.183.3.1173.
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Adoptive immunotherapy with peripheral blood lymphocytes cocultured in vitro with autologous tumor cells and interleukin-2.采用与自体肿瘤细胞及白细胞介素-2体外共培养的外周血淋巴细胞进行过继性免疫治疗。
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The tyrosinase gene codes for an antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas.酪氨酸酶基因编码一种抗原，该抗原可被HLA - A2黑色素瘤上的自体细胞溶解性T淋巴细胞识别。
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9
Sequence and expression pattern of the human MAGE2 gene.人类MAGE2基因的序列与表达模式。
Immunogenetics. 1994;39(2):121-9. doi: 10.1007/BF00188615.
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Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes.人类基因MAGE - 3编码一种抗原，该抗原可被自体溶细胞性T淋巴细胞识别，存在于黑色素瘤中。
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本文引用的文献

1
Lysis of fresh human solid tumors by autologous lymphocytes activated in vitro with lectins.用凝集素体外激活的自体淋巴细胞对新鲜人实体瘤的溶解作用。
Cancer Res. 1982 Mar;42(3):913-8.
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Lysis of fresh and cultured autologous tumor by human lymphocytes cultured in T-cell growth factor.在T细胞生长因子中培养的人淋巴细胞对新鲜的和培养的自体肿瘤的溶解作用。
Cancer Res. 1981 Nov;41(11 Pt 1):4420-5.
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Purification and some characteristics of human T-cell growth factor from phytohemagglutinin-stimulated lymphocyte-conditioned media.从植物血凝素刺激的淋巴细胞条件培养基中纯化人T细胞生长因子及其某些特性
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6134-8. doi: 10.1073/pnas.77.10.6134.
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Spontaneous in vitro cytotoxicity against autochthonous human melanoma cells.对自体人黑色素瘤细胞的自发体外细胞毒性。
J Immunol. 1980 Jan;124(1):412-7.
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Role of alloantigens in natural killing. Allogeneic but not autologous tumor biopsy cells are sensitive for interferon-induced cytotoxicity of human blood lymphcoytes.同种异体抗原在自然杀伤中的作用。同种异体而非自体肿瘤活检细胞对人血淋巴细胞的干扰素诱导细胞毒性敏感。
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A rapid method for the isolation of functional thymus-derived murine lymphocytes.一种分离功能性胸腺来源的小鼠淋巴细胞的快速方法。
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Variables and specificity of in vitro lymphocyte-mediated cytotoxicity in human melanoma.人黑色素瘤体外淋巴细胞介导的细胞毒性的变量与特异性
Cancer Res. 1975 Dec;35(12):3721-30.
8
Studies on cytotoxicity generated in human mixed lymphocyte cultures. I. Time course and target spectrum of several distinct concomitant cytotoxic activities.人类混合淋巴细胞培养中产生的细胞毒性研究。I. 几种不同伴随细胞毒性活性的时间进程和靶细胞谱。
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9
Cytotoxic effects of antigen- and mitogen-induced T cells on various targets.抗原和丝裂原诱导的T细胞对各种靶标的细胞毒性作用。
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针对人类黑色素瘤的细胞毒性T细胞反应的克隆分析。

Clonal analysis of cytotoxic T cell response against human melanoma.

作者信息

Mukherji B, MacAlister T J

出版信息

J Exp Med. 1983 Jul 1;158(1):240-5. doi: 10.1084/jem.158.1.240.

DOI:10.1084/jem.158.1.240
PMID:6602860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2187080/
Abstract

We investigated the feasibility of generating cytotoxic T cell clones against autologous human melanoma cells using a melanoma cell line (VIP) and a spontaneously transformed autologous fibroblast line (VIP-F:T). Cytotoxic lymphocytes (CL) generated against the VIP melanoma cells in one-way mixed lymphocyte-tumor cell interactions were expanded in interleukin 2 for 2 wk. The expanded CL were cloned in limiting dilution. Two phenotypically homogeneous clones (3:1 and E.5) were obtained bearing OKT3 phenotype. Both clones expressed cytotoxicity selectively only against the sensitizing autologous target VIP. cytotoxicity assays performed with clone E.5 against the VIP target cells in the presence of autologous unfractionated lymphocytes or serum showed no modulation of autoreactivity of clone E.5. These results indicate that analysis of cellular immune response against autologous tumor cells might be feasible using autoreactive clones generated by the currently available in vitro cloning technology.

摘要

我们研究了使用黑色素瘤细胞系（VIP）和自发转化的自体成纤维细胞系（VIP-F:T）产生针对自体人黑色素瘤细胞的细胞毒性T细胞克隆的可行性。在单向混合淋巴细胞-肿瘤细胞相互作用中针对VIP黑色素瘤细胞产生的细胞毒性淋巴细胞（CL）在白细胞介素2中扩增2周。将扩增的CL进行有限稀释克隆。获得了两个表型均一的克隆（3:1和E.5），它们具有OKT3表型。两个克隆均仅对致敏的自体靶细胞VIP选择性地表达细胞毒性。在存在自体未分级淋巴细胞或血清的情况下，用克隆E.5对VIP靶细胞进行的细胞毒性测定显示克隆E.5的自身反应性没有调节。这些结果表明，使用当前可用的体外克隆技术产生的自身反应性克隆来分析针对自体肿瘤细胞的细胞免疫反应可能是可行的。