北美豹蛙幼体的腹膜渗出液含有在胚胎学上源自背外侧中胚层的细胞。

Peritoneal exudate in larval Rana pipiens contains cells that are embryologically derived from dorsal lateral plate mesoderm.

作者信息

Turpen J B, Marrion R M, Williams K

出版信息

Dev Comp Immunol. 1983 Spring;7(2):295-302. doi: 10.1016/0145-305x(83)90011-3.

Abstract

The embryonic origin of cells functioning in a peritoneal exudate response of larval Rana pipiens was investigated. Normal larva received injections of sodium caseinate on day 0 and cells in the peritoneal cavity were assayed on days 3, 5, 7, 9, 11, 13 and 16. Experimental animals that received cytogenetically labeled transplants of dorsal mesoderm at 67-72 hours of development were injected with sodium caseinate on day 0 and assayed on days 5, 7 and 9. Feulgen-DNA microdensitometric analysis showed that the peritoneal exudate from days 5, 7, 9 contained hemopoietic cells that were derived from the embryonic transplant. Therefore, dorsal mesoderm gave rise to cells capable of functioning in a peritoneal exudate response to injections of sodium caseinate.

摘要

对幼体牛蛙腹膜渗出液反应中发挥作用的细胞的胚胎起源进行了研究。正常幼体在第0天注射酪蛋白酸钠，并在第3、5、7、9、11、13和16天对腹腔内的细胞进行检测。在发育67 - 72小时时接受细胞遗传学标记的背中胚层移植的实验动物，在第0天注射酪蛋白酸钠，并在第5、7和9天进行检测。福尔根DNA显微密度测定分析表明，第5、7、9天的腹膜渗出液中含有源自胚胎移植的造血细胞。因此，背中胚层产生了能够在对酪蛋白酸钠注射的腹膜渗出液反应中发挥作用的细胞。