Ward J A, Hunter C N, Jones O T
Biochem J. 1983 Jun 15;212(3):783-90. doi: 10.1042/bj2120783.
Several strains and mutants of Rhodopseudomonas sphaeroides can be grown anaerobically in the dark in the presence of dimethyl sulphoxide as an electron acceptor. During adaptation to this fermentative mode of growth, two major c-type cytochromes are synthesized, one with Mr 45 000 and the second with Mr 20 000 and a midpoint potential of +120 mV. These cytochromes are barely detectable in membranes prepared from cells grown in aerobic or photosynthetic conditions. An electrophoretic method is presented for the detection of the b-type and c-type cytochromes of pigmented or unpigmented membranes. The method resolves three b-type cytochromes and four c-type cytochromes in membranes from aerobically and photosynthetically grown cells.
球形红假单胞菌的几种菌株和突变体能够在黑暗中厌氧生长,以二甲基亚砜作为电子受体。在适应这种发酵生长模式的过程中,会合成两种主要的c型细胞色素,一种分子量为45000,另一种分子量为20000,中点电位为 +120 mV。在有氧或光合条件下生长的细胞制备的膜中,这些细胞色素几乎检测不到。本文介绍了一种电泳方法,用于检测有色或无色膜中的b型和c型细胞色素。该方法可分辨出有氧和光合生长细胞的膜中的三种b型细胞色素和四种c型细胞色素。