McEwan A G, Ferguson S J, Jackson J B
Arch Microbiol. 1983 Dec;136(4):300-5. doi: 10.1007/BF00425221.
Under dark and essentially anaerobic conditions electron flow to either dimethylsulphoxide or trimethylamine-N-oxide in cells of Rhodopseudomonas capsulata has been shown to generate a membrane potential. This conclusion is based on the observation of a red shift in the carotenoid absorption band which is a well characterised indicator of membrane potential in this bacterium. The magnitude of the dimethylsulphoxide- or trimethylamine-N-oxide-dependent membrane potential was reduced either by a protonophore uncoupler of oxidative phosphorylation or synergistically by a combination of a protonophore plus rotenone, an inhibitor of electron flow from NADH dehydrogenase. These findings, together with the observation that venturicidin, an inhibitor of the proton translocating ATPase, did not reduce the membrane potential, show that electron flow to dimethylsulphoxide or trimethylamine-N-oxide is coupled to proton translocation. Thus contrary to some previous proposals dark and anaerobic growth of Rps. capsulata in the presence of dimethylsulphoxide or trimethylamine-N-oxide cannot be regarded as purely fermentative.
在黑暗且基本厌氧的条件下，已证明在荚膜红假单胞菌细胞中，电子流向二甲基亚砜或三甲胺 - N - 氧化物会产生膜电位。这一结论基于对类胡萝卜素吸收带红移的观察，而红移是该细菌中膜电位的一个特征明确的指标。依赖于二甲基亚砜或三甲胺 - N - 氧化物的膜电位大小，要么被氧化磷酸化的质子载体解偶联剂降低，要么被质子载体加鱼藤酮（一种抑制电子从NADH脱氢酶流动的抑制剂）协同降低。这些发现，连同质子转运ATP酶抑制剂venturicidin不会降低膜电位这一观察结果，表明电子流向二甲基亚砜或三甲胺 - N - 氧化物与质子转运相偶联。因此，与之前的一些观点相反，在二甲基亚砜或三甲胺 - N - 氧化物存在的情况下，荚膜红假单胞菌的黑暗厌氧生长不能被视为纯粹的发酵过程。
