Burlington H, Cronkite E P, Heldman B, Pappas N, Shadduck R K
Blood. 1983 Sep;62(3):693-6.
A transplantable granulocytosis-inducing mammary adenocarcinoma of mice was used to provide evidence about the role of tumor-generated factors in granulopoiesis. The original tumor produced high levels of colony-stimulating factor (CSF) in culture as well as inhibitor to CSF. The tumor was passaged repeatedly, both in host mice and in culture, and eventually displayed a varying capacity to induce granulocytosis. Tumors that were associated with either the induction of extreme granulocytosis or near-normal granulocyte levels were selected and passaged intermittently in vivo and in culture. Two tumor lines were thus isolated: one, line C-4a, inducing granulocytosis, and the other, line 34-4H, failing to induce granulocytosis. Both lines grow at the same rate in host mice, but in culture, each displays a distinct morphology. Measurement of CSF and inhibitor produced by each line in culture showed that line 34-4H retained the capacity to produce CSF and inhibitor in spite of losing the ability to influence granulopoiesis in vivo. This suggests that the various factors shown to influence granulopoiesis in vitro may have little or no role as physiologic regulators in vivo.
利用一种可移植的诱导小鼠粒细胞增多的乳腺腺癌，来提供有关肿瘤产生的因子在粒细胞生成中作用的证据。原始肿瘤在培养物中产生高水平的集落刺激因子（CSF）以及CSF抑制剂。该肿瘤在宿主小鼠和培养物中反复传代，最终显示出诱导粒细胞增多的能力各不相同。选择与极端粒细胞增多诱导或接近正常粒细胞水平相关的肿瘤，并在体内和培养物中间歇传代。由此分离出两个肿瘤系：一个是诱导粒细胞增多的C-4a系，另一个是不诱导粒细胞增多的34-4H系。两个系在宿主小鼠中生长速度相同，但在培养物中，每个系都呈现出独特的形态。对每个系在培养物中产生的CSF和抑制剂的测量表明，尽管34-4H系失去了在体内影响粒细胞生成的能力，但仍保留了产生CSF和抑制剂的能力。这表明，在体外显示出影响粒细胞生成的各种因子，在体内作为生理调节因子可能作用很小或没有作用。
