Yamasaki T, Handa H, Yamashita J, Namba Y, Hanaoka M
Cancer Res. 1983 Oct;43(10):4610-7.
Immunological responses to an experimental brain tumor of mice [the 20-methylcholanthrene-induced malignant glioma, 203-glioma)] were investigated. The killer T-cell activity of spleen cells, which was specific against 203-glioma cells, began to be severely impaired 2 weeks after intracranial inoculation; this impairment was concurrent with increased intracranial pressure, which was due to developing tumor growth. On the other hand, the killer T-cell activity continued for over 4 weeks in mice inoculated with the mitomycin C-treated tumor cells. Surface marker analysis showed that Lyt-1-2,3+ killer T-cells were predominant in intracranial tumor-bearing mice, whereas both Lyt-1-,2,3+ and Lyt-1+,2,3+ killer T-cells were equally present in s.c. tumor-bearing mice. The effects of adult thymectomy on the immune responses against 203-glioma were also investigated in intracranial and s.c. tumor-bearing mice. In both the intracranially and s.c. inoculated groups, killer T-cell activity was increased in mice thymectomized before 3 weeks and decreased in mice thymectomized before 10 weeks. In these mice, Lyt-1+,2,3+ killer T-cells were not detected, which suggests strongly that the progenitors of Lyt-1+,2,3+ killer T-cells are short-lived lymphocytes in contrast to those of Lyt-1-,2,3+ killer T-cells, which survive more than 10 weeks after adult thymectomy.
对小鼠实验性脑肿瘤（20-甲基胆蒽诱导的恶性胶质瘤，203-胶质瘤）的免疫反应进行了研究。脾细胞针对203-胶质瘤细胞的杀伤性T细胞活性在颅内接种后2周开始严重受损；这种损伤与颅内压升高同时出现，颅内压升高是由于肿瘤生长所致。另一方面，在用丝裂霉素C处理过的肿瘤细胞接种的小鼠中，杀伤性T细胞活性持续了4周以上。表面标志物分析表明，在颅内荷瘤小鼠中，Lyt-1-2,3+杀伤性T细胞占主导，而在皮下荷瘤小鼠中，Lyt-1-,2,3+和Lyt-1+,2,3+杀伤性T细胞均等量存在。还在颅内和皮下荷瘤小鼠中研究了成年胸腺切除对针对203-胶质瘤的免疫反应的影响。在颅内接种组和皮下接种组中，3周前接受胸腺切除的小鼠杀伤性T细胞活性增加，而10周前接受胸腺切除的小鼠杀伤性T细胞活性降低。在这些小鼠中，未检测到Lyt-1+,2,3+杀伤性T细胞，这强烈表明，与Lyt-1-,2,3+杀伤性T细胞的祖细胞不同，Lyt-1+,2,3+杀伤性T细胞的祖细胞是寿命较短的淋巴细胞，Lyt-1-,2,3+杀伤性T细胞的祖细胞在成年胸腺切除后存活超过10周。
