Yamasaki T, Kikuchi H, Yamashita J, Handa H, Kuwata S, Taguchi M, Namba Y, Hanaoka M
Department of Neurosurgery, Medical School, Kyoto University, Japan.
Cancer Res. 1988 Jun 1;48(11):2981-7.
The effects of interleukin 2 (IL2) and interferon (IFN) on the generation and lytic activation of syngeneic murine malignant glioma (a methylcholanthrene-induced ependymoblastoma of C57BL/6 mouse origin, 203-glioma)-specific cytotoxic T-lymphocyte (G-CTL) were investigated. The surface marker analysis showed that G-CTLs from both intracranial and s.c. tumor-bearing mice were composed of thymectomy-resistant (mature) Lyt-1-.2.3+ and thymectomy-sensitive (immature) Lyt-1+.2.3+ CTLs, which markedly decreased concurrently with increased intracranial pressure. G-CTLs were confirmed to be activated with target specificity by both factors in a different way. The CTL activation by IL2 (20 units/ml) remained for a longer time, although a lag time of 5 days after initial culture was required. IL2 influenced Lyt-1+.2.3+ CTLs to proliferate and develop the lytic potential. In contrast, even a 3-h incubation with IFN (1000 units/ml) could enhance the cytotoxicity, but the augmenting effects were observed no longer than 5 days later. IFN activated Lyt-1-.2.3+ CTLs and increased their proportion of the total cell population with a simultaneous decrease of Lyt-1+.2.3+ CTLs. Therefore, it was suggested that IL2 may provide a growth of CTL populations and that IFN can accelerate recruitment of new effectors, causing activation of the lytic process.
研究了白细胞介素2（IL2）和干扰素（IFN）对同基因小鼠恶性胶质瘤（一种由甲基胆蒽诱导的源自C57BL/6小鼠的室管膜母细胞瘤，203-胶质瘤）特异性细胞毒性T淋巴细胞（G-CTL）的产生和溶解激活的影响。表面标志物分析表明，来自颅内和皮下荷瘤小鼠的G-CTL由抗胸腺切除（成熟）的Lyt-1-.2.3+和对胸腺切除敏感（不成熟）的Lyt-1+.2.3+CTL组成，它们随着颅内压升高而同时显著减少。证实这两种因子以不同方式激活具有靶标特异性的G-CTL。IL2（20单位/毫升）对CTL的激活持续时间更长，尽管初始培养后需要5天的延迟时间。IL2影响Lyt-1+.2.3+CTL增殖并发展溶解潜能。相反，即使与IFN（1000单位/毫升）孵育3小时也能增强细胞毒性，但增强作用在5天后就不再观察到。IFN激活Lyt-1-.2.3+CTL并增加它们在总细胞群体中的比例，同时Lyt-1+.2.3+CTL比例下降。因此，提示IL2可能促进CTL群体的生长，而IFN可加速新效应细胞的募集，从而导致溶解过程的激活。
