Raichle M E, Martin W R, Herscovitch P, Mintun M A, Markham J
J Nucl Med. 1983 Sep;24(9):790-8.
We have adapted the well-known tissue autoradiographic technique for the measurement of regional cerebral blood flow (CBF), originally proposed by Kety and his colleagues, for the measurement of CBF in human subjects using positron emission tomography (PET) and intravenously administered oxygen-15-labeled water. This report describes the steps necessary for the implementation of this PET/autoradiographic technique. In order to establish the accuracy of the method, we measured CBF with intravenously administered oxygen-15-labeled water and PET in anesthetized adult baboons and compared the results with blood flow measured by a standard tracer technique that uses residue detection of a bolus of oxygen-15-labeled water injected into the internal carotid artery. The correlation between CBF measured with PET and the true CBF for the same cerebral hemisphere was excellent. Over a blood-flow range of 10-63 ml/(min X 100 g), CBF (PET) = 0.90 CBF(true) + 0.40 (n = 23, r = 0.96, p less than 0.001). When blood flow exceeds 65 ml/(min X 100 g) CBF was progressively underestimated due to the known limitation of brain permeability to water.
我们采用了由凯蒂及其同事最初提出的用于测量局部脑血流量（CBF）的著名组织放射自显影技术，以利用正电子发射断层扫描（PET）和静脉注射氧-15标记水来测量人体受试者的CBF。本报告描述了实施这种PET/放射自显影技术所需的步骤。为了确定该方法的准确性，我们在麻醉的成年狒狒中用静脉注射氧-15标记水和PET测量CBF，并将结果与使用注入颈内动脉的氧-15标记水团注残留检测的标准示踪技术测量的血流量进行比较。用PET测量的CBF与同一脑半球的真实CBF之间的相关性非常好。在血流量范围为10 - 63 ml/（min×100 g）时，CBF（PET）= 0.90 CBF（真实值）+ 0.40（n = 23，r = 0.96，p＜0.001）。当血流量超过65 ml/（min×100 g）时，由于已知的脑对水的通透性限制，CBF逐渐被低估。
