Rieber M, Rieber M S
Cell Biol Int Rep. 1983 Aug;7(8):669-75. doi: 10.1016/0309-1651(83)90122-4.
We have used monolayers of bromodeoxyuridine (BrdU)-grown and control human epidermoid carcinoma (A431) cells to investigate the polypeptide changes resulting when cells are rounded by epidermal growth factor (EGF). Whereas no significant change was detected in Triton-soluble components, the urea-solubilized matrix fraction revealed greater levels of a 20 kd species in cells exposed to EGF, compared with the same cells not exposed to the growth factor. The corresponding urea fraction from BrdU-grown cells showed decreased levels of the 20 kd species as a result of exposure to EGF. Further evidence for a differential effect of EGF resulting from growth of the cells with the pyrimidine analog was observed in the matrix fraction soluble in SDS, which revealed a decrease in a 20 kd species resulting from exposure of control cells to EGF, with no comparable effect in BrdU-grown cells. Our results suggest that EGF induces a change in the properties of matrix-associated components of low molecular weight in an effect which appears to be modified by prior growth of cells with BrdU.
我们使用了经溴脱氧尿苷（BrdU）培养的单层细胞以及对照人表皮样癌细胞（A431）来研究当细胞被表皮生长因子（EGF）诱导变圆时所产生的多肽变化。虽然在Triton可溶成分中未检测到显著变化，但与未暴露于生长因子的相同细胞相比，在暴露于EGF的细胞中，尿素可溶的基质部分显示出更高水平的一种20kd的物质。由于暴露于EGF，来自BrdU培养细胞的相应尿素部分显示20kd物质的水平降低。在用SDS可溶的基质部分中观察到了嘧啶类似物培养细胞生长导致的EGF差异效应的进一步证据，这表明对照细胞暴露于EGF会导致一种20kd物质减少，而在BrdU培养的细胞中没有类似效应。我们的结果表明，EGF诱导低分子量基质相关成分的性质发生变化，而这种效应似乎会被细胞先前用BrdU培养所改变。
