Traba M L, Babé M, de la Piedra C, Marín A
Clin Chem. 1983 Oct;29(10):1806-7.
We describe a precise, specific method for measuring 24,25-dihydroxyvitamin D3 in human serum. A 2-mL serum sample is extracted with acetonitrile and passed through a Sep-Pak C-18 cartridge. The sample is further purified by "high-performance" liquid chromatography under isocratic conditions on a normal-phase column (Radial-Pak silica-gel cartridge), then subjected to a protein-binding assay. The mean concentration of 24,25-dihydroxyvitamin D3 in serum from 22 normal adults (measured during the spring) was 2.9 micrograms/L (SD 1.9, range 6.3-0.42 microgram/L). The intra-assay CV was 7.7%, the interassay CV 11.2%. Purification of the sample with Sep-Pak C-18 and liquid chromatography on normal plus reversed-phase columns leads to a mean value of 3.4 micrograms/L (SD 1.6 micrograms/L, n = 12), not significantly different from results with our method.
我们描述了一种精确、特异的测量人血清中24,25-二羟维生素D3的方法。取2 mL血清样本，用乙腈萃取并通过Sep-Pak C-18柱。样本在正相柱（径向Pak硅胶柱）上通过等度条件下的“高效”液相色谱进一步纯化，然后进行蛋白质结合测定。22名正常成年人（春季测量）血清中24,25-二羟维生素D3的平均浓度为2.9微克/升（标准差1.9，范围6.3 - 0.42微克/升）。批内变异系数为7.7%，批间变异系数为11.2%。用Sep-Pak C-18和正相及反相柱液相色谱法纯化样本，得到的平均值为3.4微克/升（标准差1.6微克/升，n = 12），与我们方法的结果无显著差异。
