Gordon S R, Rothstein H, Harding C V
Eur J Cell Biol. 1983 Jul;31(1):26-33.
Changes in the surface morphology of regenerating rabbit, rat and frog corneal endothelial cells in vivo have been investigated by scanning electron microscopy. In adult tissue these cells do not normally divide unless given a stimulus, such as injury. Surfaces of quiescent rabbit and rat cells are devoid of microvilli but display globular projections and surface pits up to 300 nm in diameter. However, regenerating endothelia are characterized by the appearance of microvilli which attain their greatest length when the cells are rounded. At this stage, cells also possess filopodia and broad processes. In cytokinesis, the microvilli have shortened and blebs and ruffles appear for the first time. In contrast to rabbits and rats, frog endothelial cells of noninjured tissue are covered by microvilli and smaller surface pits of 60-70 nm diameters. During regeneration, these cells have reduced numbers of microvilli and extensive foldings of the membrane. Neither blebs nor filopodia occur during the mitotic cycle and ruffles are not detected until cytokinesis.
利用扫描电子显微镜对再生过程中兔、大鼠和青蛙角膜内皮细胞的表面形态变化进行了研究。在成年组织中，这些细胞通常不会分裂，除非受到诸如损伤之类的刺激。静止状态下的兔和大鼠细胞表面没有微绒毛，但有直径达300纳米的球状突起和表面凹陷。然而，再生内皮细胞的特征是出现微绒毛，当细胞变圆时微绒毛达到最长。在此阶段，细胞还具有丝状伪足和宽大的突起。在细胞分裂时，微绒毛缩短，第一次出现小泡和褶皱。与兔和大鼠不同，未受伤组织的青蛙内皮细胞覆盖着微绒毛和直径为60 - 70纳米的较小表面凹陷。在再生过程中，这些细胞的微绒毛数量减少，膜出现广泛折叠。在有丝分裂周期中既不出现小泡也不出现丝状伪足，直到细胞分裂时才检测到褶皱。
