小鼠IgG1抗体的亲同种细胞活性和嗜异细胞活性

Homocytotropic and heterocytotropic activity of mouse IgG1 antibodies.

作者信息

Mota I, Perini A

出版信息

Int Arch Allergy Appl Immunol. 1983;72(3):199-205. doi: 10.1159/000234868.

Abstract

Mouse anti-ovalbumin antibodies were isolated by affinity chromatography and fractionated in a column of protein A-Sepharose. Three peaks were obtained: peak I that did not bind to protein A, peak II eluted with 0.5 M NaSCN and peak III eluted with 2.0 M NaSCN. Peak I and peak II contained IgG1 but no detectable IgG2 or IgG3, whereas peak II contained IgG2 and IgG3 but no detectable IgG1. Peak I, but not peak II, showed heat-resistant passive cutaneous anaphylactic (PCA) activity in rats, which was absorbed by anti-IgG1 serum but not by anti-IgE. Both peak I and peak II showed heat-resistant PCA activity in mice, that was absorbed by anti-IgG1. Peak III showed PCA activity in guinea pigs but not in mice or rats. These findings suggest that mouse IgG1 can be divided into 2 populations that differ in physicochemical and biological properties.

摘要

通过亲和层析法分离小鼠抗卵清蛋白抗体，并在蛋白A-琼脂糖柱上进行分级分离。得到了三个峰：峰I不与蛋白A结合，峰II用0.5M硫氰酸钠洗脱，峰III用2.0M硫氰酸钠洗脱。峰I和峰II含有IgG1，但未检测到IgG2或IgG3，而峰III含有IgG2和IgG3，但未检测到IgG1。峰I而非峰II在大鼠中表现出耐热性被动皮肤过敏反应（PCA）活性，该活性可被抗IgG1血清吸收，但不能被抗IgE吸收。峰I和峰II在小鼠中均表现出耐热性PCA活性，且可被抗IgG1吸收。峰III在豚鼠中表现出PCA活性，但在小鼠或大鼠中未表现出。这些发现表明，小鼠IgG1可分为两个在物理化学和生物学特性上不同的群体。