Helland S, Broch O J, Ueland P M
Neuropharmacology. 1983 Jul;22(7):915-7. doi: 10.1016/0028-3908(83)90140-5.
The tight-binding adenosine deaminase inhibitor, 2'-deoxycoformycin (dCF), was continuously infused into mice by intraperitoneal implantation of microosmotic pumps delivering the compound at a rate of 0.16 mg hr-1 kg-1 for up to 6 days. The activity of cerebral adenosine deaminase was nearly totally inhibited. The amount of adenosine and 2'-deoxyadenosine was determined in the brain frozen in liquid nitrogen through the intact skull bone. The concentration of adenosine was about 1 nmol/g, and was essentially not altered following treatment with deoxycoformycin. Deoxycoformycin induced a progressive increase in cerebral content of 2'-deoxyadenosine, which after 1 day of treatment equalled the amount of adenosine. The concentrations of serotonin, dopamine and noradrenaline in the brain were not altered.
通过腹腔植入微渗透泵以0.16毫克/小时·千克-1的速率持续向小鼠输注紧密结合型腺苷脱氨酶抑制剂2'-脱氧助间型霉素（dCF），持续时间长达6天。脑腺苷脱氨酶的活性几乎被完全抑制。通过完整的颅骨对液氮冷冻的脑组织中的腺苷和2'-脱氧腺苷含量进行测定。腺苷浓度约为1纳摩尔/克，用脱氧助间型霉素治疗后基本未改变。脱氧助间型霉素使脑内2'-脱氧腺苷含量逐渐增加，治疗1天后其含量与腺苷量相当。脑内血清素、多巴胺和去甲肾上腺素的浓度未改变。
