Weaver F A, Torkelson A R, Zygmunt W A, Browder H P
J Pharm Sci. 1978 Jul;67(7):1009-12. doi: 10.1002/jps.2600670738.
An in vitro cytotoxicity assay for cyclophosphamide metabolites in rat body fluids is described. Of the two tissue culture tumor cell lines employed, the Walker-256 rat carcinosarcoma was more sensitive to metabolite levels than the L-1210 mouse lymphocytic leukemia. The Walker-256 system detected cyclophosphamide metabolite levels two orders of magnitude lower than the commonly used 4-(p-nitrobenzyl)pyridine analytical procedure.
本文描述了一种针对大鼠体液中环磷酰胺代谢物的体外细胞毒性测定方法。在所使用的两种组织培养肿瘤细胞系中，Walker-256大鼠癌肉瘤对代谢物水平的敏感性高于L-1210小鼠淋巴细胞白血病。Walker-256系统检测到的环磷酰胺代谢物水平比常用的4-（对硝基苄基）吡啶分析方法低两个数量级。
