Tominaga A, Lefort S, Mizel S B, Dambrauskas J T, Granstein R, Lowy A, Benacerraf B, Greene M I
Clin Immunol Immunopathol. 1983 Nov;29(2):282-93. doi: 10.1016/0090-1229(83)90030-2.
In order to clarify the nature of the defect in the process of antigen presentation caused by uv radiation, low-density spleen cells were used as a potent APC source in a hapten-specific cytolytic T-cell (Tc) system. It was demonstrated that IA+ weakly adherent low-density spleen cells, when directly coupled with azobenzene arsonate (ABA), led to the activation ABA-specific Tc. When these APC were exposed to uv radiation (12 J/m2/sec) for 30 sec, their ability to lead to Tc activation was markedly inhibited. The defect imposed by uv radiation could be specifically bypassed by the addition of small amounts of homogeneous IL-1 or IL-2. This led to the specific activation of ABA-reactive H-2-restricted Tc. The purified IL-1 was also found to bypass the systemic defect imposed in vivo by external uv radiation of mice. This may indicate a potential therapeutic role for IL-1.
为了阐明紫外线辐射导致的抗原呈递过程中缺陷的本质，在半抗原特异性细胞毒性T细胞（Tc）系统中，低密度脾细胞被用作有效的抗原呈递细胞（APC）来源。结果表明，IA⁺弱黏附性低密度脾细胞与偶氮苯砷酸盐（ABA）直接偶联时，可导致ABA特异性Tc的激活。当这些APC暴露于紫外线辐射（12 J/m²/秒）30秒时，其导致Tc激活的能力受到显著抑制。添加少量同源白细胞介素-1（IL-1）或白细胞介素-2（IL-2）可特异性绕过紫外线辐射造成的缺陷。这导致了ABA反应性H-2限制性Tc的特异性激活。还发现纯化的IL-1可绕过小鼠体外紫外线辐射在体内造成的系统性缺陷。这可能表明IL-1具有潜在的治疗作用。
