Alexandrová M, Macho L
Endocrinol Exp. 1983 Oct;17(3-4):183-9.
The concentration of corticosteroids found by a competitive protein binding (CPB) assay in human milk ranged from 20 to 136 ng ml-1, in cow milk 8 to 18 ng ml-1 and rat in milk it averaged 144 ng ml-1. The lower concentration of cortisol in human milk was observed when thin layer chromatography of milk extract was made before CPB assay. Using thin layer and paper chromatography cortisone, cortisol and progesterone were identified in human milk, while in cow milk cortisol and progesterone and in rat milk only corticosterone was found. The presence of these hormones in plasma suggests a possibility of their transport from blood to the milk.
通过竞争性蛋白结合（CPB）测定法发现，人乳中皮质类固醇的浓度范围为20至136纳克/毫升，牛乳中为8至18纳克/毫升，大鼠乳中平均为144纳克/毫升。在CPB测定前对乳汁提取物进行薄层色谱分析时，观察到人乳中皮质醇的浓度较低。利用薄层色谱法和纸色谱法，在人乳中鉴定出可的松、皮质醇和孕酮，而在牛乳中鉴定出皮质醇和孕酮，在大鼠乳中仅发现皮质酮。血浆中这些激素的存在表明它们有可能从血液转运至乳汁。
