Cruickshank J K, Price K M, Mackenzie C D, Spry C J, Denham D A
Parasite Immunol. 1983 Nov;5(6):527-37. doi: 10.1111/j.1365-3024.1983.tb00769.x.
Infective larvae of Brugia pahangi were injected subcutaneously into inbred PVG (-RTIc) rats, and 'nude' (PVG-rnu/rnu) (athymic) rats. Adult worms or circulating microfilariae were recovered from 20/34 (59%) of PVG-RTIc rats and from 30/30 (100%) of 'nude' rats. Fertile worms were regularly found in the lumbar lymphatics and hearts of both strains of rat. Blood eosinophilia first developed in PVG-RTIc rats about 17 days, and in all such animals by 6 weeks. High circulating eosinophil counts persisted only in patent animals, proving a useful hallmark for the presence of microfilariae. Nude rats despite patency, developed eosinophilia only latterly and then to a lesser extent. Specific anti-B. pahangi IgG antibody was first detected at 7 days in all infected PVG-RTIc rats, with levels rising until 8 weeks and remaining high only in microfilaraemic animals; total IgE showed a similar response. Specific IgE rose in all the eight patent rats inconsistently and only to low levels in eight non-patent infected rats. IgG and IgE were undetectable in nude rats. Other strains of inbred rats of different RTI haplotype were also successfully infected with B. pahangi and the human parasite B. malayi, a total of 10/23 (43%) and 5/15 (33%) becoming patent respectively. In the small numbers tested no major influence of RTI haplotype was detected. Infection by the intraperitoneal route did not result in the development of microfilariae. The difference in patency rates between 'nude' and normal PVG rats supports the contention that the development of filarial infections is T lymphocyte dependent. Inbred and 'nude' rats provide a valuable model of human filariasis, in which many features of filarial immunopathology can be studied.
将彭亨布鲁线虫的感染性幼虫皮下注射到近交系PVG（-RTIc）大鼠和“裸”（PVG-rnu/rnu）（无胸腺）大鼠体内。在34只PVG-RTIc大鼠中有20只（59%）以及30只“裸”大鼠中的30只（100%）体内发现了成虫或循环微丝蚴。在这两种品系大鼠的腰淋巴和心脏中均经常发现有能产幼虫的成虫。PVG-RTIc大鼠大约在第17天首次出现血液嗜酸性粒细胞增多，到6周时所有此类动物均出现该现象。高循环嗜酸性粒细胞计数仅在有微丝蚴的动物中持续存在，这证明是微丝蚴存在的一个有用标志。“裸”大鼠尽管有虫血症，但嗜酸性粒细胞增多仅在后期出现，且程度较轻。在所有受感染的PVG-RTIc大鼠中，在第7天首次检测到特异性抗彭亨布鲁线虫IgG抗体，其水平一直上升至8周，并且仅在有微丝蚴血症的动物中保持高水平；总IgE呈现类似反应。在所有8只有虫血症的大鼠中，特异性IgE的升高不一致，而在8只无虫血症的受感染大鼠中仅升高至低水平。在“裸”大鼠中未检测到IgG和IgE。不同RTI单倍型的其他近交系大鼠品系也成功感染了彭亨布鲁线虫和人体寄生虫马来布鲁线虫，分别有10/23（43%）和5/15（33%）出现虫血症。在所测试的少数动物中，未检测到RTI单倍型的主要影响。经腹腔途径感染未导致微丝蚴的发育。“裸”大鼠和正常PVG大鼠在出现虫血症的比率上的差异支持丝虫感染的发展依赖于T淋巴细胞这一论点。近交系大鼠和“裸”大鼠提供了一个研究人类丝虫病的有价值模型，在该模型中可以研究丝虫免疫病理学的许多特征。
