血浆纤连蛋白-C1q复合物的形成及其对C1q抑制胶原诱导血小板聚集的影响。

Plasma fibronectin-C1q complex formation and its effect on C1q inhibition of collagen-induced platelet aggregation.

作者信息

Wautier J L, Wautier M P, Kadeva H, Mosesson M W

出版信息

Thromb Res. 1983 Nov 1;32(3):283-90. doi: 10.1016/0049-3848(83)90163-9.

Abstract

The first component of complement (C1q) contains a collagen-like structure which evidently accounts for its ability to bind to platelets and to inhibit collagen-induced platelet aggregation. This present investigation explored the interaction among C1q, plasma fibronectin (Pl Fn) and platelets. Gel filtration chromatography (Sepharose 2B and 4B) of preincubated mixtures of C1q and Pl Fn yielded evidence of complex formation. Preincubation of Pl Fn with platelets did not affect collagen-induced platelet aggregation nor did preincubation of Pl Fn with C1q significantly affect C1q-mediated inhibition of collagen-induced platelet aggregation. These observations may indicate that the site of interaction between Pl Fn and C1q is different from the site of interaction between C1q and platelets.

摘要

补体的第一成分（C1q）含有一种胶原样结构，显然这解释了其与血小板结合并抑制胶原诱导的血小板聚集的能力。本研究探讨了C1q、血浆纤连蛋白（Pl Fn）与血小板之间的相互作用。对C1q和Pl Fn的预孵育混合物进行凝胶过滤层析（琼脂糖2B和4B），结果表明形成了复合物。Pl Fn与血小板预孵育不影响胶原诱导的血小板聚集，Pl Fn与C1q预孵育也不显著影响C1q介导的对胶原诱导的血小板聚集的抑制作用。这些观察结果可能表明，Pl Fn与C1q之间的相互作用位点不同于C1q与血小板之间的相互作用位点。