Barna B P, Deodhar S D, Gautam S, Edinger M, Chiang T, McMahon J T
Int Arch Allergy Appl Immunol. 1984;73(1):49-55.
In previous studies, we found that endotracheal administration of beryllium oxide (BeO) produced cell-mediated immunity and granulomatous lung disease in strain 2 but not strain 13 guinea pigs. This report describes phenotypic and functional studies of bronchial lavage cells from both guinea pig strains after BeO exposure. In BeO-treated strain 2 animals, the percentage of T (ER+)3 lymphocytes was significantly (p less than 0.05) elevated above normal by 2 weeks after BeO exposure, while T lymphocytes were not increased in strain 13. Giant cells containing crystalline material in fused, coalesced phagolysosomes were observed in treated animals of both strains. BeO-exposed strain 2 alveolar macrophages (AM) demonstrated enhanced killing of Listeria monocytogenes and enhanced chemiluminescence, while AM of strain 13 had responses below controls. Findings support the presence of BeO-induced cellular immunologic activity in strain 2 animals and, on the other hand, suggest BeO toxicity to lavage cells in strain 13.
在先前的研究中，我们发现，给2号品系而非13号品系的豚鼠气管内注入氧化铍（BeO）可产生细胞介导免疫及肉芽肿性肺病。本报告描述了BeO暴露后两种品系豚鼠支气管灌洗细胞的表型及功能研究。在经BeO处理的2号品系动物中，BeO暴露后2周，T（ER +）3淋巴细胞百分比显著（p小于0.05）高于正常水平，而13号品系的T淋巴细胞未增加。在两个品系经处理的动物中均观察到，融合、合并的吞噬溶酶体中含有晶体物质的巨细胞。暴露于BeO的2号品系肺泡巨噬细胞（AM）对单核细胞增生李斯特菌的杀伤能力增强，化学发光增强，而13号品系的AM反应低于对照组。这些发现支持2号品系动物中存在BeO诱导的细胞免疫活性，另一方面，提示BeO对13号品系灌洗细胞具有毒性。
