下丘脑促肾上腺皮质激素释放因子的比较色谱分析。

Comparative chromatography of hypothalamic corticotrophin-releasing factors.

作者信息

Gillies G E, Puri A, Linton E A, Lowry P J

出版信息

Neuroendocrinology. 1984 Jan;38(1):17-24. doi: 10.1159/000123860.

Abstract

Extracts of rat stalk-median eminences were chromatographed on Sephadex G-50 and BioGel P2. Effluent fractions were monitored for corticotrophin-releasing factor (CRF) bioactivity, using the perfused isolated pituitary cell column bioassay, and radioimmunoassay for CRF-41, AVP, oxytocin, neurophysin, adrenocorticotrophic hormone (ACTH) and luteinizing hormone-releasing hormone (LHRH). Porcine hypothalami were also chromatographed on Sephadex G-50. Our results suggest that vasopressin and a peptide in the rat related to synthetic ovine CRF are two components of the hypothalamic CRF complex which potentiate each other at the corticotrope to release ACTH. However, these two substances alone do not appear to be wholly responsible for CRF bioactivity of hypothalamic stalk-median eminence extracts and other CRFs may yet be found.

摘要

将大鼠正中隆起提取物在葡聚糖G - 50和生物凝胶P2上进行色谱分析。使用灌注分离垂体细胞柱生物测定法监测流出级分中的促肾上腺皮质激素释放因子（CRF）生物活性，并对CRF - 41、抗利尿激素（AVP）、催产素、神经垂体素、促肾上腺皮质激素（ACTH）和促黄体生成激素释放激素（LHRH）进行放射免疫测定。猪下丘脑也在葡聚糖G - 50上进行色谱分析。我们的结果表明，抗利尿激素和大鼠中一种与合成绵羊CRF相关的肽是下丘脑CRF复合物的两个组成部分，它们在促肾上腺皮质激素细胞处相互增强以释放ACTH。然而，仅这两种物质似乎并不能完全解释下丘脑正中隆起提取物的CRF生物活性，可能还会发现其他CRF。