Olsson I L, Sarngadharan M G, Breitman T R, Gallo R C
Blood. 1984 Mar;63(3):510-7.
Mitogen-stimulated mononuclear blood cells produce differentiation inducing factors (DIFs) for the promyelocytic cell line HL-60. We report that DIF is produced constitutively by a malignant T lymphocyte line HUT-102. DIF was purified 7,000-fold from HUT-102 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography, and preparative SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The final preparation is susceptible to protease treatment, has a molecular weight of 46,000, as determined by SDS-PAGE and approximately 55,000 by gel filtration, has an isoelectric point of approximately 5.2, does not adhere to lectin-Sepharose and is resistant to periodate oxidation, and is free of colony-stimulating factor. DIF induced maturation of HL-60 into phagocytizing nitro blue tetrazolium reducing cells with the morphological characteristics of myelomonocytic or monocyte-like cells. An activity, co-chromatographing with DIF, acts synergistically with retinoic acid to induce maturation not only of HL-60, but also of the monoblast-like cell line U-937 (measured as percentage of cells reducing NBT).
有丝分裂原刺激的单核血细胞可产生针对早幼粒细胞系HL-60的分化诱导因子（DIF）。我们报告，恶性T淋巴细胞系HUT-102可组成性产生DIF。利用DEAE-琼脂糖离子交换色谱、凝胶色谱、蓝色琼脂糖色谱和制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），从HUT-102条件培养基中纯化出7000倍的DIF。最终制品对蛋白酶处理敏感，经SDS-PAGE测定分子量为46,000，经凝胶过滤测定约为55,000，等电点约为5.2，不黏附于凝集素-琼脂糖且对高碘酸盐氧化有抗性，并且不含集落刺激因子。DIF可诱导HL-60成熟为具有髓单核细胞或单核细胞样细胞形态特征的吞噬硝基蓝四氮唑还原细胞。一种与DIF共色谱的活性物质，可与视黄酸协同作用，不仅诱导HL-60成熟，还可诱导单核母细胞样细胞系U-937成熟（以还原NBT的细胞百分比衡量）。
