Stachura I, Si L, Madan E, Whiteside T
Clin Immunol Immunopathol. 1984 Mar;30(3):362-73. doi: 10.1016/0090-1229(84)90022-9.
Mononuclear inflammatory cells (MIC) in renal biopsies from 37 patients with renal disease were studied by avidin--biotin--immunoperoxidase complex (ABC) technique, utilizing monoclonal antibodies to cell surface antigens: T11 (total T), T4 (inducer/helper), T8 (suppressor/cytotoxic), B1 (B cells), M1 (monocytes), and Leu-7 (natural killer, NK cells). Renal MIC consisted mostly of T cells and monocytes. T cells were a predominating cell type in the renal interstitium of all patients studied (64-88% of MIC). The T4:T8 ratios ranged from 0.4 +/- 0.3 (mean +/- SEM) in interstitial nephritis to 2.5 +/- 0.9 in membranous glomerulonephritis. M1+ cells constituted from 10 to 62% of glomerular MIC and from 5 to 24% of interstitial MIC. Glomerular MIC were rare or absent in patients with IgA nephropathy (IgA N). These results support the concept that in situ interactions of T lymphocytes and monocytes may modulate the events leading to the development of human renal disease. The striking absence of glomerular MIC in IgA N could be related to persistence of immune deposits in the glomeruli of patients with this renal disorder.
采用抗生物素蛋白-生物素-免疫过氧化物酶复合物（ABC）技术，利用针对细胞表面抗原的单克隆抗体：T11（总T细胞）、T4（诱导/辅助细胞）、T8（抑制/细胞毒性细胞）、B1（B细胞）、M1（单核细胞）和Leu-7（自然杀伤细胞，NK细胞），对37例肾病患者肾活检中的单核炎性细胞（MIC）进行了研究。肾MIC主要由T细胞和单核细胞组成。在所有研究患者的肾间质中，T细胞是主要的细胞类型（占MIC的64% - 88%）。T4:T8比值在间质性肾炎中为0.4±0.3（平均值±标准误），在膜性肾小球肾炎中为2.5±0.9。M1 +细胞占肾小球MIC的10% - 62%，占肾间质MIC的5% - 24%。IgA肾病（IgA N）患者的肾小球MIC罕见或不存在。这些结果支持这样一种概念，即T淋巴细胞和单核细胞的原位相互作用可能调节导致人类肾病发生的事件。IgA N中肾小球MIC的显著缺失可能与该肾脏疾病患者肾小球中免疫沉积物的持续存在有关。
