Yasuhara M, Kimura T, Sezaki H
J Pharmacobiodyn. 1983 Nov;6(11):888-91. doi: 10.1248/bpb1978.6.888.
Brush border membrane vesicles of rat small intestinal mucosa were prepared by the modified calcium precipitation method in order to investigate the intestinal secretion mechanism of p-acetamidobenzoic acid (Ac-PABA). The release rate of [14C]-Ac-PABA from preloaded vesicles was much faster than that of p-aminobenzoic acid (PABA) and was enhanced by the addition of unlabelled Ac-PABA in the outer medium. In contrast, the membrane transfer rate of Ac-PABA across the egg lecithin liposomes was slower than that of PABA. The contribution of a carrier-mediated transport mechanism in the brush border membrane to the intestinal secretion of Ac-PABA was suggested.
