论黏液纤毛流动的力学原理。III. 蛙腭黏液中的流速剖面

On the mechanics of mucociliary flows. III. Flow-velocity profiles in frog palate mucus.

作者信息

Winet H, Yates G T, Wu T Y, Head J

出版信息

J Appl Physiol Respir Environ Exerc Physiol. 1984 Mar;56(3):785-94. doi: 10.1152/jappl.1984.56.3.785.

DOI:10.1152/jappl.1984.56.3.785

Abstract

Flow-velocity profiles over excised frog ciliated epithelium were obtained for the region within about 600 micron of the mucosa. Fluorescent particles were used as flow tracers. Both a control and an autologous mucus suspension were observed. The control culture medium was bounded by the walls of the observation chamber, and mucus was deposited on the epithelium as a blob after mixing it with tracers. In spite of the difference in boundary conditions the two profiles, normalized to maximum particle velocity and solution depth, were indistinguishable at heights over 60 micron from the mucosa. The near-mucosa profiles in contrast were unalike with mucus exhibiting a greater shear gradient than the control culture medium. It was concluded that ciliary contact is not necessary for generation of mucus flow provided the ciliary shear is not negated by the mucus "flake" or "slab" being in simultaneous contact with significant ciliostatic patches which would act as anchors.

摘要

在切除的青蛙纤毛上皮上，获取了距黏膜约600微米范围内区域的流速剖面。使用荧光颗粒作为流动示踪剂。观察了对照和自体黏液悬浮液。对照培养基由观察室壁界定，黏液与示踪剂混合后作为一个团块沉积在上皮上。尽管边界条件不同，但将两个剖面归一化到最大颗粒速度和溶液深度后，在距黏膜60微米以上的高度处无法区分。相比之下，近黏膜剖面不同，黏液表现出比对照培养基更大的剪切梯度。得出的结论是，只要黏液“薄片”或“板”不同时与作为锚的显著纤毛静止斑块接触而使纤毛剪切不被抵消，纤毛接触对于黏液流动的产生不是必需的。