Huang Brendan K, Choma Michael A
Department of Biomedical Engineering, Yale University, New Haven, USA,
Cell Mol Life Sci. 2015 Mar;72(6):1095-113. doi: 10.1007/s00018-014-1784-z. Epub 2014 Nov 23.
Cilia-driven fluid flow is important for multiple processes in the body, including respiratory mucus clearance, gamete transport in the oviduct, right-left patterning in the embryonic node, and cerebrospinal fluid circulation. Multiple imaging techniques have been applied toward quantifying ciliary flow. Here, we review common velocimetry methods of quantifying fluid flow. We then discuss four important optical modalities, including light microscopy, epifluorescence, confocal microscopy, and optical coherence tomography, that have been used to investigate cilia-driven flow.
纤毛驱动的流体流动对身体的多个过程都很重要,包括呼吸道黏液清除、输卵管中的配子运输、胚胎节点的左右模式形成以及脑脊液循环。多种成像技术已被用于量化纤毛流动。在这里,我们回顾了量化流体流动的常用测速方法。然后,我们讨论了四种重要的光学模态,包括用于研究纤毛驱动流动的光学显微镜、落射荧光显微镜、共聚焦显微镜和光学相干断层扫描。