Lampen J O
Experientia Suppl. 1983;46:243-7. doi: 10.1007/978-3-0348-6776-4_28.
The changing nature of the research reported at these Symposia over the past two decades is noted, especially the current emphasis on plant protoplasts. Improvements in procedures for the preparation and regeneration of microbial protoplasts are summarized and the definition of optimal conditions for fusion and genetic transfer. The efficiency of fusion now attainable has led to considerable advances in the genetics of yeasts and mycelial fungi and of bacteria. In addition, the synthesis and secretion of beta-lactamase (penicillinase) in Bacillus licheniformis is described in order to illustrate how protoplasts can be utilized to investigate 1) the form in which the new enzyme emerges from the membrane surface, 2) the importance of accessibility in processing of the emerging chain and 3) the role of cell wall structure in the induction of beta-lactamase synthesis.
有人指出了过去二十年来在这些研讨会上所报告研究性质的变化，尤其是当前对植物原生质体的重视。总结了微生物原生质体制备和再生程序的改进以及融合和基因转移最佳条件的定义。目前可实现的融合效率已在酵母、丝状真菌和细菌遗传学方面取得了相当大的进展。此外，还描述了地衣芽孢杆菌中β-内酰胺酶（青霉素酶）的合成与分泌，以说明原生质体如何可用于研究：1）新酶从膜表面出现的形式；2）新生肽链加工过程中可及性的重要性；3）细胞壁结构在β-内酰胺酶合成诱导中的作用。
