Goers J W, Glovsky M M, Hunkapiller M W, Farnsworth V, Richards J H
Int Arch Allergy Appl Immunol. 1984;74(2):147-51. doi: 10.1159/000233535.
C3a purified to chemical homogeneity from human serum binds preferentially to human eosinophils greater than neutrophils. Little or no binding is found with human platelets. Maximum binding to eosinophils at 37 degrees C occurs within 15 min. Dilution of 125I-C3a by either cold C3a or washing away unbound 125I-C3a and reincubating at 37 degrees C reveals a T1/2 of approximately 30 min. C3adesArg neither binds to eosinophils nor inhibits the binding of 125I-C3a. The binding of C3a to human eosinophils may reflect a physiologic role of C3a in eosinophil motility or function.
从人血清中纯化至化学纯的C3a优先与人嗜酸性粒细胞结合，其结合程度大于中性粒细胞。与人血小板几乎没有或没有结合。在37℃时，与嗜酸性粒细胞的最大结合在15分钟内发生。用冷C3a稀释125I-C3a，或洗去未结合的125I-C3a并在37℃下重新孵育，显示半衰期约为30分钟。C3adesArg既不与嗜酸性粒细胞结合，也不抑制125I-C3a的结合。C3a与人嗜酸性粒细胞的结合可能反映了C3a在嗜酸性粒细胞运动或功能中的生理作用。
