Koizumi S, Yamagami M, Horita S, Miura M, Sano M, Ikuta N, Taniguchi N
Int J Cell Cloning. 1983 Jul;1(3):130-41. doi: 10.1002/stem.5530010301.
The present study was undertaken to elucidate the role of T cell subsets in regulating the in vitro growth of human granulopoietic progenitor cells (CFU-C). Prior to CFU-C assay, mononuclear cells obtained from bone marrow and cord blood were depleted of T cells or functionally distinct T cell subsets by the method of complement-mediated cytolysis with the use of monoclonal antibodies, OKT3, OKT4 and OKT8. The depletion of any T cell subset of OKT3+, OKT4+ or OKT8+ cells from bone marrow and cord blood cells showed no significant alterations in the generation of CFU-C. The supplementation to the in vitro culture system of OKT4+ or OKT8+ cells, which had been negatively selected by complement-mediated cytolysis using the mutually exclusive monoclonal OKT8 or OKT4 antibody, respectively, did not alter the growth of CFU-C-derived colonies without mitogenic stimulation. In contrast, the production of colony-stimulating activity (CSA) in peripheral blood mononuclear cells was significantly reduced by removing not only OKT3+ cells but also OKT4+ or OKT8+ cell subsets. There were no significant differences in the degree of reduction between the different procedures. These results suggested that T cell subsets played an important role in the regulation of steady-state granulopoiesis through the stimulation of CSA production. The presence of a specific subset of T cells in CSA production was not demonstrated.
本研究旨在阐明T细胞亚群在调节人粒细胞祖细胞（CFU-C）体外生长中的作用。在进行CFU-C测定之前，通过使用单克隆抗体OKT3、OKT4和OKT8，采用补体介导的细胞溶解方法，从骨髓和脐血中获得的单核细胞去除T细胞或功能不同的T细胞亚群。从骨髓和脐血细胞中去除任何OKT3 +、OKT4 +或OKT8 +细胞的T细胞亚群，CFU-C的生成均未显示出明显变化。分别用相互排斥的单克隆OKT8或OKT4抗体通过补体介导的细胞溶解进行阴性选择的OKT4 +或OKT8 +细胞补充到体外培养系统中，在无促有丝分裂刺激的情况下，并未改变CFU-C衍生集落的生长。相反，通过不仅去除OKT3 +细胞，而且去除OKT4 +或OKT8 +细胞亚群，外周血单核细胞中的集落刺激活性（CSA）产生显著降低。不同程序之间的降低程度没有显著差异。这些结果表明，T细胞亚群通过刺激CSA产生在稳态粒细胞生成的调节中起重要作用。未证明CSA产生中存在特定的T细胞亚群。
