Harris W R, Friedman S B, Silberman D
J Inorg Biochem. 1984 Feb;20(2):157-69. doi: 10.1016/0162-0134(84)80015-x.
Radiolabeled vanadium as either vanadyl ion or vanadate ion was injected intravenously into adult beagle dogs, and blood samples were collected at various times up to 48 hr post injection. For each sample, the distribution of vanadium between the cells and the plasma was determined, and the plasma was analyzed by electrophoresis to identify specific vanadium-binding proteins. Initially, vanadyl ion left the bloodstream more rapidly than vanadate, but the rates equalized after about 5 hr. A significant fraction of the vanadium in blood was associated with the cellular component following injection of both forms of vanadium. About 77% of the plasma vanadium was eventually bound by the serum iron transport protein transferrin, regardless of the vanadium species initially injected. For both vanadyl and vanadate, about 30 hr were required to reach the maximum degree of transferrin binding.
将放射性标记的钒以钒离子或钒酸盐离子的形式静脉注射到成年比格犬体内，并在注射后长达48小时的不同时间采集血样。对于每个样本，测定细胞和血浆之间钒的分布，并通过电泳分析血浆以鉴定特定的钒结合蛋白。最初，钒离子比钒酸盐更快地离开血流，但约5小时后速率趋于相等。注射两种形式的钒后，血液中很大一部分钒与细胞成分相关。无论最初注射的钒种类如何，约77%的血浆钒最终被血清铁转运蛋白转铁蛋白结合。对于钒离子和钒酸盐，达到转铁蛋白结合的最大程度都需要约30小时。
