使用新型荧光底物对血清中人类胰腺和唾液α淀粉酶进行差异测定。

Differential assay of human pancreatic and salivary alpha-amylases in serum using a new fluorogenic substrate.

作者信息

Omichi K, Ikenaka T

出版信息

Clin Chim Acta. 1984 Apr 13;138(2):197-203. doi: 10.1016/0009-8981(84)90234-1.

Abstract

The difference in the mode action of human pancreatic and salivary alpha-amylases on O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1----4)-O-alpha-D - glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-D-glucitol FG6R), a fluorogenic derivative of maltohexaitol, was found. The products of the enzymatic hydrolysis were analyzed by high-performance liquid chromatography (HPLC) in 8 min. FG6R was hydrolyzed by these enzymes to O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl- (1----4)-O-alpha-D-glucopyranosyl-(1----4)-D-glucose (FG3) and maltotriitol, or O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D- glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-D-glucose (FG4) and maltitol. Pancreatic alpha-amylase produced more FG4 than salivary alpha-amylase. Taking advantage of the differences in action of the two amylases, a differential alpha-amylase assay in serum was performed. The method is simple and rapid and can be used for routine clinical assays of alpha-amylases.

摘要

人们发现了人胰腺α-淀粉酶和唾液α-淀粉酶对O-6-脱氧-6-[(2-吡啶基)氨基]-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-D-葡萄糖醇（FG6R，麦芽六糖醇的一种荧光衍生物）的作用方式差异。通过高效液相色谱法（HPLC）在8分钟内对酶促水解产物进行了分析。FG6R被这些酶水解为O-6-脱氧-6-[(2-吡啶基)氨基]-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-D-葡萄糖（FG3）和麦芽三糖醇，或O-6-脱氧-6-[(2-吡啶基)氨基]-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-O-α-D-吡喃葡萄糖基-(1→4)-D-葡萄糖（FG4）和麦芽糖醇。胰腺α-淀粉酶产生的FG4比唾液α-淀粉酶更多。利用这两种淀粉酶作用的差异，进行了血清中α-淀粉酶的差异测定。该方法简单快速，可用于α-淀粉酶的常规临床检测。