人胰腺α淀粉酶和唾液α淀粉酶在转糖基化方面的差异。

Difference in transglycosylation between human pancreatic and salivary alpha-amylases.

作者信息

Omichi K, Ikenaka T

出版信息

J Biochem. 1983 Dec;94(6):1797-802. doi: 10.1093/oxfordjournals.jbchem.a134531.

DOI:10.1093/oxfordjournals.jbchem.a134531

Abstract

Transglycosylation reactions of alpha-amylases from human pancreatic juice and saliva were examined by using O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-D-glucopyranose as a substrate and O-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-1-deoxy-1-[(2-pyridyl)amino]-D-glucitol as an acceptor. The transfer reaction was estimated by quantitation of O-alpha-D-glucopyranosyl-(1 leads to 4)-1-deoxy-1-[(2-pyridyl)amino]-D-glucitol produced by the enzymes from the transfer products, because the acceptor was not hydrolyzed. The amount of O-alpha-D-glucopyranosyl-(1 leads to 4)-1-deoxy-1-[(2-pyridyl)amino]-D-glucitol in the digest with pancreatic alpha-amylase was six times that in the digest with salivary alpha-amylase at the stage when the substrate was completely consumed, and the difference increased gradually on further incubation. The phenomenon can be applied to differentiate the two alpha-amylases in human serum.

摘要

以O - 6 - 脱氧 - 6 - [(2 - 吡啶基)氨基] - α - D - 吡喃葡萄糖基 - (1→4) - O - α - D - 吡喃葡萄糖基 - (1→4) - O - α - D - 吡喃葡萄糖基 - (1→4) - O - α - D - 吡喃葡萄糖基 - (1→4) - D - 吡喃葡萄糖作为底物，O - α - D - 吡喃葡萄糖基 - (1→4) - O - α - D - 吡喃葡萄糖基 - (1→4) - 1 - 脱氧 - 1 - [(2 - 吡啶基)氨基] - D - 葡萄糖醇作为受体，检测了人胰液和唾液中α - 淀粉酶的转糖基化反应。由于受体不会被水解，所以通过定量酶从转糖基化产物中产生的O - α - D - 吡喃葡萄糖基 - (1→4) - 1 - 脱氧 - 1 - [(2 - 吡啶基)氨基] - D - 葡萄糖醇来评估转糖基化反应。在底物完全消耗的阶段，胰α - 淀粉酶消化液中O - α - D - 吡喃葡萄糖基 - (1→4) - 1 - 脱氧 - 1 - [(2 - 吡啶基)氨基] - D - 葡萄糖醇的量是唾液α - 淀粉酶消化液中的6倍，并且在进一步孵育时差异逐渐增大。该现象可用于区分人血清中的两种α - 淀粉酶。