Koishi T, Minowada J, Henderson E S, Ohnuma T
Gan. 1984 Mar;75(3):275-83.
Forty-two human hematopoietic cell lines were assessed for sensitivity to a four-day incubation with L-asparaginase. Eight of 13 T-leukemia cell lines and 1 of 7 non-T, non-B common acute lymphoblastic leukemia cell lines exhibited an ID50 (50% growth inhibition dose) of less than 0.0001 IU/ml. Seven of these sensitive cell lines were further cultured in L-asparagine-free medium and were found not to proliferate. Five T-leukemia cell lines, 6 non-T, non-B common acute lymphoblastic leukemia cell lines, 10 "abnormal" B-cell lines, 4 "normal" B-cell lines, 3 myeloma cell lines and 5 myeloid leukemia cell lines had ID50 values between 0.1 and 1.0 IU/ml. Twelve of these resistant cell lines were able to proliferate in L-asparagine-depleted medium. The results indicate that some patients with T-cell acute lymphoblastic leukemia might respond to this enzymatic antineoplastic drug, L-asparaginase, at low dose.
评估了42种人类造血细胞系对L-天冬酰胺酶四天孵育的敏感性。13种T淋巴细胞白血病细胞系中的8种以及7种非T、非B普通急性淋巴细胞白血病细胞系中的1种，其半数抑制生长剂量（ID50）小于0.0001 IU/ml。将其中7种敏感细胞系在无L-天冬酰胺的培养基中进一步培养，发现其无法增殖。5种T淋巴细胞白血病细胞系、6种非T、非B普通急性淋巴细胞白血病细胞系、10种“异常”B细胞系、4种“正常”B细胞系、3种骨髓瘤细胞系和5种髓性白血病细胞系的ID50值在0.1至1.0 IU/ml之间。这些耐药细胞系中有12种能够在缺乏L-天冬酰胺的培养基中增殖。结果表明，一些T细胞急性淋巴细胞白血病患者可能对这种酶类抗肿瘤药物L-天冬酰胺酶低剂量治疗有反应。
