Nagata K, Platt J L, Michael A F
Kidney Int. 1984 Jan;25(1):88-93. doi: 10.1038/ki.1984.12.
Tissue samples from patients with idiopathic nephrotic syndrome [seven with minimal change (MC) and seven with mesangial proliferation (MP)] were examined for evidence of interstitial and glomerular immune cell infiltration using monoclonal antibodies to identify T cells (OKT3, TA-1), T cell subsets (OKT4, OKT8), B cells (BA-1), and monocytes and null cells (OKM1) by indirect immunofluorescence. A nuclear counterstain permitted precise enumeration of reactive and unreactive cells in comparison with five normal tissue samples, eight endstage (ES) tissue samples, and five tissue samples from patients with miscellaneous ( misc ) types of nephrotic syndrome. Interstitial cell populations in MC and MP were similar to normals except for an increase in OKM1 reactive cells in MP. ES had more numerous interstitial cells reactive with each of the monoclonal antibodies than did normal, MC, or MP. Reactive glomerular cells were most numerous in MP, intermediate in MC but only rarely observed in normal tissue samples. Most identifiable glomerular cells reacted with OKT3 or OKM1. T-cell subset populations in MC and MP glomeruli were similar. These studies reveal the presence of infiltrating immune cells in the glomeruli but not the renal interstitium of patients with idiopathic nephrotic syndrome. Whether these cells mediate proteinuria or simply mark tissue injury remains to be discerned.
对特发性肾病综合征患者的组织样本（7例微小病变型肾病患者和7例系膜增生性肾病患者）进行检查，使用单克隆抗体通过间接免疫荧光法鉴定T细胞（OKT3、TA-1）、T细胞亚群（OKT4、OKT8）、B细胞（BA-1）以及单核细胞和裸细胞（OKM1），以寻找间质和肾小球免疫细胞浸润的证据。通过核复染，与5份正常组织样本、8份终末期（ES）组织样本以及5份其他（misc）类型肾病综合征患者的组织样本相比，可精确计数反应性和非反应性细胞。微小病变型肾病和系膜增生性肾病中的间质细胞群体与正常情况相似，只是系膜增生性肾病中OKM1反应性细胞有所增加。终末期肾病的间质细胞与每种单克隆抗体反应的数量均多于正常、微小病变型肾病或系膜增生性肾病。反应性肾小球细胞在系膜增生性肾病中最多，微小病变型肾病中居中，而在正常组织样本中很少见到。大多数可识别的肾小球细胞与OKT3或OKM1反应。微小病变型肾病和系膜增生性肾病肾小球中的T细胞亚群群体相似。这些研究揭示了特发性肾病综合征患者肾小球中存在浸润性免疫细胞，但肾间质中不存在。这些细胞是介导蛋白尿还是仅仅标记组织损伤仍有待确定。
