Romslo I
Clin Chim Acta. 1977 Feb 15;75(1):171-4. doi: 10.1016/0009-8981(77)90515-0.
When Tb3+ ions are incubated with human serum, there is a large enhancement in the fluorescence of the Tb3+ ions as revealed by the excitation and emission wavelength pair 288 and 545 nm. The fluorescence enhancement represents the binding of Tb3+ ions not only to (apo)transferrin, but to albumin and gamma-globulins as well. Spectrophotometric evidence indicate involvement of tyrosyl residues at the cation binding site(s). The ion fluorescence induced by albumin and gamma-globulins invalidates the usefulness of the fluorometric determination of transferrin in the routine laboratory.
当Tb3+离子与人血清一起孵育时，如通过激发和发射波长对288和545nm所显示的，Tb3+离子的荧光有很大增强。荧光增强不仅代表Tb3+离子与（脱铁）转铁蛋白的结合，也代表与白蛋白和γ-球蛋白的结合。分光光度法证据表明阳离子结合位点处的酪氨酸残基参与其中。白蛋白和γ-球蛋白诱导的离子荧光使常规实验室中荧光法测定转铁蛋白的实用性失效。
