采用高效液相色谱法对产甲烷菌中的辅酶及相关化合物进行定量分析。

Quantification of coenzymes and related compounds from methanogenic bacteria by high-performance liquid chromatography.

作者信息

Van Beelen P, Geerts W J, Pol A, Vogels G D

出版信息

Anal Biochem. 1983 Jun;131(2):285-90. doi: 10.1016/0003-2697(83)90171-9.

Abstract

Quantification of coenzymes and related compounds from methanogens was performed in extracts obtained from whole cells with aqueous ethanol at 80 degrees C. By means of high-performance liquid chromatography the following compounds could be detected and quantified in extracts from Methanobacterium thermoautotrophicum: coenzyme MF430, the prosthetic group of methylcoenzyme M reductase, F560, an oxidation product of this compound, coenzyme F420, F342, methanopterin, and carboxytetrahydromethanopterin, previously known as YFC. Coenzyme MF430, coenzyme F420, and methanopterin could be determined in extracts from Methanosarcina barkeri. Structural differences were noticed between the coenzymes from the methanogenic bacteria studied.

摘要

利用80℃的含水乙醇从全细胞中提取提取物，对产甲烷菌中的辅酶及相关化合物进行了定量分析。通过高效液相色谱法，可在嗜热自养甲烷杆菌的提取物中检测并定量以下化合物：甲基辅酶M还原酶的辅基辅酶MF430、该化合物的氧化产物F560、辅酶F420、F342、甲蝶呤以及羧基四氢甲蝶呤（以前称为YFC）。在巴氏甲烷八叠球菌的提取物中也可测定辅酶MF430、辅酶F420和甲蝶呤。在所研究的产甲烷细菌的辅酶之间发现了结构差异。