van de Wijngaard W M, vermey P, van der Drift C
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
J Bacteriol. 1991 Apr;173(8):2710-1. doi: 10.1128/jb.173.8.2710-2711.1991.
Cell extracts of Methanosarcina barkeri converted coenzyme F420 in an ATP-dependent reaction to the adenylylated derivative factor 390. Although it was reported previously (L. M. Gloss and R. P. Hausinger, BioFactors 1:237-240, 1988) that whole cells were unable to perform this conversion, we observed the conversion in 7 of 11 extracts, all of which were prepared from different batches of cells.
巴氏甲烷八叠球菌的细胞提取物在依赖ATP的反应中将辅酶F420转化为腺苷酸化衍生物因子390。尽管此前有报道(L.M.格洛斯和R.P.豪辛格,《生物因子》1:237 - 240,1988年)称完整细胞无法进行这种转化,但我们在11种提取物中的7种中观察到了这种转化,所有这些提取物均来自不同批次的细胞。