Berkos O V
Fiziol Zh SSSR Im I M Sechenova. 1976 Nov;62(11):1661-6.
Antigenic properties of crystalline pepsin, trypsin and chymotrypsin were studied in 9 rabbits immunised with these enzymes. Production of antibodies and their titres were investigated by means of the reaction of the precipitation in testing tubes and immunoelectrophoresis. The most clear antigenic properties were revealed in trypsin, and they were the weakest in pepsin. Low titres of the antibody to pepsin were, probably, due to denaturation of its molecule at physiological values of pH immunisation. Absence of cross-reactions of antisera to trypsin and chymotrypsin with pepsin solutions may be due to considerable structural differences of the antigenic determinants of pepsin from those of trypsin and chemotrypsin. However, antisera to trypsin and chemotrypsin do develop cross-reactions with respective enzymes and there were may be due to a similarity between structures of their antigenic determinants.
在9只经结晶胃蛋白酶、胰蛋白酶和胰凝乳蛋白酶免疫的兔子中研究了这些酶的抗原特性。通过试管沉淀反应和免疫电泳研究抗体的产生及其滴度。胰蛋白酶显示出最明显的抗原特性，而胃蛋白酶的抗原特性最弱。胃蛋白酶抗体滴度低可能是由于其分子在免疫时的生理pH值下发生了变性。抗胰蛋白酶和抗胰凝乳蛋白酶血清与胃蛋白酶溶液无交叉反应，可能是由于胃蛋白酶抗原决定簇的结构与胰蛋白酶和胰凝乳蛋白酶的抗原决定簇有很大差异。然而，抗胰蛋白酶和抗胰凝乳蛋白酶血清确实会与各自的酶发生交叉反应，这可能是由于它们抗原决定簇的结构相似。
