Antigens on boar spermatozoa and the effect of antibodies against the spermatozoa and the accessory sexual gland fluids on boar spermatozoa.

Antigens on the acrosomes of boar spermatozoa could be detected by the anti-complement immunofluorescence test, using antisera against spermatozoa and against seminal vesicle and epididymis fluids. Weak fluorescence was observed even on the tail and no fluorescence in the postnuclear segment. Adequate absorption of the complement allowed the sperm-immobilizing test to be used with boar spermatozoa. Using this test, the specificity of the sperm-immobilizing effect on antispermatozoal antibodies was confirmed. The sperm-immobilizing effect and damage to the acrosomes was prevented by absorption of the sera with spermatozoa. Antibodies against the fluid of the boar prostate did not react with antigens on boar spermatozoa.